Monoclonal Antibody to Microcystin-LR 微囊藻毒素單克隆抗體
Product Specifications
CLONE: MC8C10
ISOTYPE: Mouse IgG2b
IMMUNOGEN : Microcystin-LR linked via N-methyl-dehydroalanine to bovine serum albumin (BSA).
SPECIFICITY : Recognizes Microcystin-LR, the other [4-arginine] microcystins and the nodularin-R
showed lower cross-reactivates (CR ≤10%), and other MCs such as MC-LF and MC-LW
are not recognized (CR < 10?4 ).
APPLICATION : ELISA (1:8'000-1:10'000) Microcystin concentrations can be measured in the range of
0.1μg/L to 10.0μg/L.
PURITY DETAIL: Protein A-affinity purified.
FORMULATION : Solution in PBS buffer.
SHIPPING : SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY : Storage at 4°C will decrease antibody activity and must be avoided.
HANDLING : Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Description
With a indirect competitive enzyme-linked immunosorbent assay (ELISA) using antibody MC8C10, IC50 values for microcystin-LR of 1.8 ±0.1μg/Lhave been obtained. The detection limit for microcystin-LR is 0.1μg/L. The provisional guideline value proposed by the WHO is 1 μg/L for drinking water. All microcystin variants containing an arginine at position 4 (except microcystin-LR) show larger IC50 values (CR ≤10%) and detection limits, whereas other microcystins, such as microcystin-LA are not recognized. The affinity constant for MC8C10 was determined to be at least 2.8x108 L/mol. The antibody was tested for its robustness against interferences (humic acids, pH, salt content, surfactants or organic solvents) and was found to be very stable. Microcystin-LR spiked water samples in the concentration of 3.0μg/L were measured and a mean recovery of 92%–105.3% was found. The antibody is well suited for the sensitive analysis of microcystins in drinking as well as surface water.