CRM-ASP-Mus

Mussel Tissue Certified Reference Material for Domoic Acid

Domoic Acid (DA) is a toxin responsible for incidents of amnesic shellfish poisoning (ASP) on the east and west coasts of North America [1,2]. CRM-ASP-Mus-d is a mussel tissue (Mytilus edulis) homogenate containing DA, as well as several related compounds (Figures 1 and 2). This is a replacement CRM for the depleted product CRM-ASP-Mus-c which was released in 2005. The tissue

was thermally stabilized, homogenized, heat sealed in polypropylene bottles and packed in trilaminate pouches. Each pouch contains two 4 g bottles of homogenate with DA at levels appropriate for analytical testing. The concentration of DA and the epimeric C5’-epi-DA in CRM-ASP-Mus-d was assigned using a high recovery extraction method and quantitation by liquid chromatography with both

UV absorbance and mass spectrometric detection (Table 1).

Table 1: Certified concentration value for CRM-ASP-Mus

Expiry date: 1 year from date of sale

Storage conditions: -12 °C or below

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