* Limited stock available.  If stock is not available, Cytoskeleton will produce a new batch upon request.  Minimum order will apply.  Inquire for more information.

Product Uses

• Measurement of phosphate in buffers or serum.

• Identification/characterization of ATPase proteins.

• Measurement of enzymatic parameters of ATPases and GTPases.

Materials

The purine nucleoside phosphorylase protein has been produced in a bacterial expression system.  The recombinant protein (aa1-283) contains a 6xHIS-tag at the amino terminus and has a combined calculated molecular weight of approximately 32 kDa.  The protein has been determined to be biologically active in a microtubule-activated phosphate transfer assay (see below).  The protein is supplied as a white lyophilized powder.