Product Uses Include

• To study the effects of pharmaceutical compounds on the ratio of G-actin to F-actin.

• To study the effects of mutated cell lines versus their parent cell line for the change in ratio of G-actin to F-actin.

• To study the effects of physical alterations of environment on the ratio of G-actin to F-actin. 
  
  

Introduction
The most reproducible and accurate method of determining the amount of filamentous actin (F-actin) content versus free globular-actin (G-actin) content in a cell population is to use Western blot quantitation of F-actin and G-actin cellular fractions (1-4).  The general approach is to homogenize cells in F-actin stabilization buffer, followed by centrifugation to separate the F-actin from G-actin pool. The fractions are then separated by SDS-PAGE and actin is quantitated by Western blot. The final result gives the most accurate method of determining the ratio of F-actin incorporated into the cytoskeleton versus the G-actin found in the cytosol. This kit contains all the reagents needed to perform this assay.