Anti-ubiquitin-HRP antibody is a pan-ubiquitin, mouse monoclonal antibody conjugated with HRP that is part of the Signal-Seeker? product line. The antibody was raised against full length bovine ubiquitin. The antibody has been shown to recognize poly-ubiquitin, mono-ubiquitin, and free ubiquitin (Fig 1). Ubiquitin is a highly conserved protein and AUB01 is predicted to recognize ubiquitin from a wide range of species (5). AUB01-HRP has been validated in western blot applications and has been shown to be more potent than unconjugated AUB01 used with standard mouse-HRP secondary. AUB01 is purified by Protein G affinity chromatography and is supplied as a lyophilized white powder.