Product uses

• Identification and characterization of FtsZ binding proteins

• Characterization of FtsZ dynamics

• Developing FtsZ ligands that may be used as anti-bacterial agents.
  
  

Material
FtsZ is a bacterial cytoskeletal protein that is essential for cell division many prokaryotes (1).  It has been shown to be a bacterial homolog of eukaryotic tubulin, based both on a low sequence identity and a striking structural similarity (2).  Just like eukaryotic tubulin, FtsZ polymerizes as well as binds and hydrolyzes GTP in a polymerization dependent manner.

E. coli FtsZ protein has been purified after over-expression in E.coli. E.c. FtsZ has a 6xHis tag and an approximate molecular weight of 45 kDa (Fig. 1). FTZ05 is provided as a lyophilized protein with buffer and stabilizers.

Purity
Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 10% polyacrylamide gel. ?FtsZ protein was calculated to be >90% pure (see Figure 1, Lane 2).