The BlastR? Buffer system allow efficient extraction of proteins from all cellular compartments (Fig. 1). BlastR? lysis buffer system is compatible with standard colorimetric protein assays unlike Laemmli and 1% denaturing lysis buffer (Fig. 3) It should be noted that BlastR? Buffer will efficiently break up protein:protein interactions and is therefore not suitable for co-IP applications.
NOTE: Lysates may become viscous due to the release of genomic DNA. The Lysate viscosity can be eliminated after passage over a BlastR? filter (not included in this kit), providing a user friendly lysate for downstream applications including SDS-PAGE, western blotting, immunoprecipiatations (IPs). The BlastR? filters are sold separately and can be used with any nuclear lysis buffer system to reduce lysate viscosity by removing genomic DNA from the sample (Fig. 2)
Product Uses Include
Remove genomic DNA
Suitable for SDS-PAGE Application
Suitable for IP Applications
Sample Kit Components (50 lyses)
BlastR? Lysis buffer
BlastR? Dilution buffer