Product Uses

• Study of K-Ras4B exchange activity with different GEFs.

• Identification of K-Ras4B exchange factors (GEFs)

• Positive control for GEF studies.

• Biochemical characterization of K-Ras4B protein interactions

• Western blot standard

Materials

The wild-type human K-Ras4B protein has been produced in a bacterial expression system.  The  recombinant protein contains six histidine residues at its amino terminus (His-tag).  The molecular weight of 6xHis tagged K-Ras4B is approximately 25 kDa and is supplied as a white lyophilized powder.

Storage

Before reconstitution, briefly centrifuge to collect the product at the bottom of the tube.  The protein should be reconstituted to 5 mg/ml with the addition of 20 μl of Milli-Q water (100 μg size). ?When reconstituted, the protein will be in the following buffer: 50 mM Tris pH 7.5, 50 mM NaCl, 0.5 mM MgCl2, 5% (w/v) sucrose and 1%  (w/v) dextran.  In order to maintain high biological activity of the protein it is strongly recommended that the protein solution be supplemented with DTT to 1 mM final concentration, aliquoted into  "experiment sized" amounts, snap frozen in liquid nitrogen and stored a -70°C. The protein is stable for six months if stored at -70°C. The protein should not be exposed to repeated freeze-thaw cycles.  The lyophilized protein is stable at 4°C desiccated (<10% humidity) for one year.