Heavy Meromyosin Protein (HMM Fragment) 重鏈肌球蛋白（HMM片段）

零售價(jià): 詢(xún)價(jià) 品牌：Cytoskeleton 產(chǎn)品編號(hào)：CS-MH03 等級(jí)：蛋白規(guī)格：1 x 100 μg CAS No：

* 干冰運(yùn)輸、大包裝及大批量的產(chǎn)品需酌情添加運(yùn)輸費(fèi)用

* 零售價(jià)、促銷(xiāo)產(chǎn)品折扣、運(yùn)輸費(fèi)用、庫(kù)存情況、產(chǎn)品及包裝規(guī)格可能因各種原因有所變動(dòng)，恕不另行通知，確切詳情請(qǐng)聯(lián)系北京伊普瑞斯技術(shù)開(kāi)發(fā)有限公司中國(guó)客服

詳細(xì)信息

Product Uses

Measurement of calcium activated cardiac HMM ATPase activity when bound to thin filaments.
Identification/characterization of proteins or small molecules that affect the TT complex regulation and cardiac HMM ATPase activity
Identification/characterization of proteins or small molecules that affect cardiac HMM / F- actin interaction

Materials

Cardiac myosin protein has been purified from bovine heart tissue (1,2,3). The full length myosin protein was purified with its essential light chains (ELC) and regulatory light chains (RLC), see Figure 1 and 2. Myosin was then digested with a-chymotrypsin in the presence of MgCl2 to liberate the soluble heavy fragment (HMM) domain, which was isolated by centrifugation followed by anionic exchange chromatography to remove S1 (2,3). The purified myosin HMM fragment has been determined to be biologically active in an F-actin activated ATPase assay (see biological activity assay). Bovine cardiac myosin S1 fragment protein is supplied as a white lyophilized powder.

Figure 1. Diagrammatic representation of the myosin protein and its subfragments