Product Uses

• Study inhibitors of SOS2 GTP exchange activity

• Biochemical characterization of SOS2 specific protein interactions

• Study of SOS2 GEF activity with different GTPases

• Compare differences in SOS1 and SOS2 exchange activities

Materials

The human SOS2 exchange domain (563-1051) protein (SOS2-ExD) has been produced in a bacterial expression system.  The recombinant protein contains a  6x histidine tag (His-tag) at its amino terminus.  The approximate molecular weight of the SOS2-ExD protein is 61 kDa.  The protein is supplied as a lyophilized white powder.

Storage and Reconstitution

The protein should be reconstituted to 50 mM (3.03 mg/ml) by the addition of 33 μl of nanopure water. ?The protein will be in the following buffer; 17 mM Tris pH 7.5, 17 mM NaCl, 0.2 mM MgCl2, 2.3% sucrose, 0.3% dextran.  In order to maintain high biological activity of the protein, it is strongly recommended that the protein solution  should be aliquoted into "experiment sized" aliquots and snap frozen in liquid nitrogen. The protein can be stored at -70°C for 6 months.  Avoid repeated freeze-thaw cycles.  The lyophilized protein is stable at 4°C for 1 year when stored desiccated (<10% humidity).