Material
Pre-formed actin filaments (PAFs) are supplied as a lyophilized powder. PAFs have been prepared from rabbit skeletal muscle actin protein that is greater than 99% pure (Cat. # AKL99). These stringently quality controlled filaments provide highly reliable and  reproducible results in assays that require actin filament substrates. The average filament length in this product is 8 μm. Using these PAFs in assays depending on filaments gives very reproducible results. In an actin filament activated myosin ATPase assay (see Fig. 1), the CV between samples (Vmax) was less than 5% and the CV of Vmax between assays using different batches of AKF99 was less than 8%.