Product Uses Include
Identification and characterization of cardiac actin binding proteins
In vitro cardiac actin polymerization studies
Material:
Bovine cardiac muscle actin is supplied as a lyophilized powder. When one vial is reconstituted in 1 ml of Actin Monomer Buffer: 5.0 mM Tris-HCI pH 8.0, 0.2 mM CaCl2, 0.2 mM ATP and 0.5 mM DTT, it is at a final concentration of 1.0 mg/ml.
Purity:
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Samples are > 99% pure.
Biological Activity:
The biological activity is determined in three ways. The first method utilizes pyrene muscle actin as a fluorescent indicator of polymerization (use a 1:10 ratio of AP07 to AD99) . The second involves sedimentation of actin polymer by high speed centrifugation. The third method measures the integrity of the monomer by the specific inhibition of DNase activity. The biological activity is > 90%.