Product Uses

• Measurement of calcium activated myosin ATPase activity when bound to thin filaments.

• Identification/characterization of proteins or small moleculesthat affect the CTFC regulation and myosin ATPase activity

• Identification/characterization of proteins or small molecules that affect myosin / F-actin interaction

Materials

The cardiac thin filament complex (CTFC) has been assembled from purified F-actin (Cat. # AD99) and Tropomyosin / Troponin protein (TT) complex (Cat. # TT05). Thus, CTFC is composed of six proteins: Actin : Tropomyosin α?: Tropomyosin β : Troponin C :Troponin I : Troponin T in a stoichiometric ratio of 7:1:1:1:1:1; see Figure 1. After assembly, the thin filaments are centrifuged at 100,000 x g and the pellets resuspended in reaction buffer which purifies the calcium sensitive complex. The complex has been determined to be biologically active in a calcium activated myosin ATPase assay (see biological activity assay). The complex is supplied as a white lyophilized powder.