Product Uses Include
Positive control for the study of microtubule binding proteins
Investigation of the the effect of Tau on microtubule dynamics
Material
Tau protein is isolated from MAP-rich bovine brain tubulin by ion exchange chromatography over a phosphocellulose matrix. The protein is supplied lyophilized. When reconstituted to 1 mg/ml (protein concentration determined by Precision Red Advanced Protein Assay, Cat. # ADV02), the protein will be in in 50 mM PIPES, 1 mM EDTA and 16 mM NaCl.
Purity
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Samples are approximately 90% Tau proteins. Approximatley 1% contamination by high molecular weight MAPs and 9% other MAPs and non-MAP proteins.
Figure 1: Tau protein purity determination. A 10 μg sample of TA01 was separated by electrophoresis in a 12% SDS-PAGE system, and stained with Coomassie Blue.
Biological Activity