Product Uses Include

• RhoA biochemistry

• RhoA GTPase assays

• RhoA nucleotide exchange assays

• RhoA binding studies
  
  

Material
The human RhoA protein has been produced in a bacterial expression system. The protein is supplied as a lyophilized powder. When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent, Cat. # ADV02.

The recombinant protein is approximately 27 kDa, consisting of the RhoA protein plus a histidine tag in the amino-terminus.

For other forms of RhoA as well as many other purified small G-proteins, see our main small G-protein product page.

Purity
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. His-RhoA samples are >80% pure.