Product Uses Include

• RhoA effector and GAP identification and binding studies

• Activation of RhoA effectors in vitro and in vivo

• Stimulation of RhoA phenotype by microinjection into cells
  
  

Material
The constitutively active form of human RhoA protein contains a glutamine to leucine substitution at residue 63. The common name for this mutant is RhoA(Q63L) or L63RhoA. The leucine substitution prevents endogenous and GAP-stimulated GTPase activity of RhoA, hence the protein is always in the active, GTP-bound, state. The corresponding mutation in Ras (Ras(Q61L)) behaves as an oncogenic mutation.