PAK Beads, Rac1/Cdc42 binding domain protein on beads PAK珠，Rac1/Cdc42結合域蛋白

零售價: 詢價品牌：Cytoskeleton 產(chǎn)品編號：PAK02-A 等級：蛋白規(guī)格：1 x 500 μg CAS No：

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詳細信息

Product Uses Include

Measurement of the GTP/GDP ratio of Rac or Cdc42 in vitro.
Quantitation of GTP-Rac/Cdc42 from tissue and tissue culture cell lysates.

Material
The Rac/Cdc42 (p21) binding domain (PBD) of the human p21 activated kinase 1 protein (PAK) protein has been expressed as a GST-fusion protein in E. coli. This protein binds binds specifically to GTP-bound, and not GDP-bound, Rac and Cdc42 proteins. The domain can therefore be used to specifically precipitate active, GTP-bound Rac and Cdc42 as well as to specifically block the activity of Rac and Cdc42 in vitro and in vivo.

The GST-PAK-PBD contains residues 67-150 of PAK. This region includes the highly conserved CRIB motif plus sequences required for the high affinity interaction with GTP-Rac and GTP-Cdc42.

The protein is supplied in a glutathione agarose bound format and is shipped lyophilized. The beads are colored for ease of use. This product is used in our Cdc42 and Rac activation assay Biochem Kits? (Cat. # BK034 and BK035, repectively). The GST-PAK-PBD is also available as a free protein (Cat. # PAK01).